This course is part of a series taught by Kevin Ahern at Oregon State University on General Biochemistry. For more information about online courses go to ecampus.oregonstate.edu for the rest of the courses see www.youtube.com 1. The chemiosmotic hypothesis, originally proposed by Peter Mitchell, explains how mitochondria make ATP in oxidative phosphorylation. Important aspects of it include: a. Intact inner mitochondrial membrane b. Electron transport creates a proton gradient c. ATP is made by movement of protons back into the mitochondria 2. Coupling of electron transport and oxidative phosphorylation at a practical level means that the mitochondrial inner membrane remains impermeable to protons, except for those that enter via the ATP synthase and result in ATP production. 3. The ATP synthase consists of a turbine-like structure containing 3 sites called Loose (L), Tight (T), and Open (O). Functions of these forms include L – Holds ADP and Pi in preparation for ATP formation O – Releases ATP formed in T and binds ADP + Pi T – Causes ADP and Pi to join and form ATP 4. Movement of protons through the ATP synthase cases rotation/conformational changes in the complex that result in formation of ATP from ADP and Pi. Conversions in the process occur as follows: O goes to L L goes to T T goes to O 5. Mitochondria which are “tightly coupled” have intact membranes AND the only way protons get back into the matrix is by passing through Complex V. If you poke a hole in the …
Under this sun no shadows will fall Piercing our eyes as we charge An armoured battalion on course to the east Closing the end of it’s march This time we’re here to finish a job Started a decade ago Driving the animals out of their holes To bury them 6 feet below Armoured tanks of mass destruction Killers in the east Rats who dares to stand before us Feel our guns go live Death in the shape of a panzer battalion Insect of terror don’t run face your fate like a Man cannot outrun our panzer battalion Thousands of tons of armour and guns Making it’s way through the sand Our panzer battalion is back for revenge Artillery sweeping the land First strike is ours no mercy is shown There’s rivers of blood in our track Breaking their lines of defence with our tanks Infantry watching our back Blow their SAM sites clear for air strike Ready for the storm Minefields swept there’s no surrender Feel our napalm burn Under this sun no shadows will fall Piercing our eyes as we charge An armoured battalion on course to the east Has reached the end of it’s march Video Rating: 4 / 5
